[Content determination] According to HPLC determination.
Chromatographic conditions and system suitability test: Use ODS as filler; methanol-0.05% phosphoric acid solution (22:78) as mobile phase; detection wavelength as 237nm. The number of theoretical plates should not be less than 5,000 calculated based on the peak of Swertiamarin.
Preparation of the reference solution: Take an appropriate amount of Swertiamarin reference substance, accurately weigh it, then add methanol to make a solution containing 0.1 mg per 1 ml.
Preparation of the test solution: Take 0.5g of the powder (passed through No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, add 50ml of methanol accurately, weigh it, and ultrasonically treat it (power 250W, frequency 40kHZ) ) 30 minutes, let it cool, then check the weight, use methanol to make up the lost weight, shake, filter, accurately measure 5ml of the filtrate, place it in a 25ml measuring flask, add methanol to the mark, shake, filter, take the continuous filtrate and get it.
Detection method: Precisely draw 5-10ul each of the reference solution and test solution, and inject it into the HPLC instrument for determination.
This product is calculated as a dry product, and contains no less than 8.0% Swertiamarin (C16H22O10).