【Content Detection】
Preparation of Wolfberry Polysaccharide Reference Solution:
Take 25mg reference substance of anhydrous glucose, accurately weigh it, put it in a 250ml measuring bottle, add some water to dissolve, dilute to the mark, shake well, and get it (each l ml contains anhydrous glucose with 0.1mg). Preparation of the standard curve: Precisely measure the reference solution with 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml and separately place them in the test tube with stopper, add water to make up to 2.0ml, and each precisely add 1ml of 5% phenol solution, shake well, quickly and accurately add 5ml sulfuric acid, shake well, waiting for 10 minutes, then keep it in a 40°C water bath for 15 minutes, take it out, quickly cool to room temperature, then use the corresponding reagent as a blank, and follow the ultraviolet-visible spectrophotometry, measure the absorbance at a wavelength of 490nm, then with absorbance as the ordinate and concentration as the abscissa, and draw a standard curve.
Detection Method:
Take about 0.5g coarse powder of this product, accurately weigh it, and add 100ml ether, heat to reflux for 1 hour, then stand still and let cool, carefully discard the ether solution, and place the residue on a water bath to evaporate the ether. Then add 100ml of 80% ethanol, heat to reflux for 1 hour, filter while hot, wash the filter residue and filter with 30ml of hot 80% ethanol, then put the filter residue and filter paper in the flask, add 150ml water, and heat to reflux for 2 hours. Then filter while hot, and wash the filter with a small amount of hot water, then combine the filtrate and lotion, let cool and transfer to a 250ml measuring flask, dilute to the mark with water, shake well, accurately measure 1ml, then place it in a test tube with a stopper and add 1.0ml water. According to the method under the preparation of the standard curve, starting from "each precisly add 1ml of 5% phenol solution", measure the absorbance accordingly, and read the weight (mg) of glucose contained in the test solution from the standard curve, calculate and get it.
Caculated as a dried product, the content of polysaccharides in wolfberry as glucose (C6H12O6), which should not be less than 1.8%.
Betaine:
Cut this product into small pieces, take about 2g, accurately weigh it, then add 50ml of 80% methanol, heat to reflux for 1 hour, then let cool and filter, and then wash the residue and filter with 30ml of 80% methanol, combine the lotion and filtrate, and concentrate to 10ml, adjust the pH value to 1 with hydrochloric acid, then add 1g activated carbon, heat to boil, let cool and filter, then wash with 15ml water, combine the lotion and the filtrate, and then add 20ml of the newly prepared solution with 2.5% chromium ammonium thiocyanate, stir well, and place under 10°C for 3 hours. Filter with a G4 vertical melting funnel, wash the precipitate with a small amount of ice water, drain it, and dissolve the residue with acetone, then transfer it to a 5ml measuring flask, add acetone to the mark, shake well, and use it as the test solution. In addition, take an appropriate amount of betaine reference substance, accurately weigh it, and add hydrochloric acid methanol solution (0.5-100) to make a solution containing 4 mg per 1 ml as a reference substance solution. According to thin layer chromatography test, accurately draw 5ul of the test solution, 3ul and 6ul of the reference solution, and crossly place them on the same silica gel G thin-layer plate. Take acetone-absolute ethanol-hydrochloric acid (10:6:1) as a developing agent, pre-saturate for 30 minutes, unfold, then take out and evaporate the solvent, then immediately spray with the newly prepared test solution of modified potassium bismuth iodide , leave it for 1 to 3 hours until the spots are clear. Scan according to thin layer chromatography, with wavelength as λS=515nm and λR=590nm, measure the integral value of absorbance of the test substance and the reference substance, calculate, and get it.
Calculated as a dried product, the content of betaine (C5H11NO2) shall not be less than 0.30%.