[Content determination] Mangiferin is determined by HPLC.
Chromatographic conditions and system suitability test:use ODS as filler; acetonitrile-0.2% glacial acetic acid aqueous solution (15:85) as mobile phase; detection wavelength as 258nm.The number of theoretical plates should not be less than 6000 calculated on the mangiferin peak.
Preparation of reference substance solution: Take an appropriate amount of mangiferin reference substance, accurately weigh it, add dilute ethanol to make a solution containing 50μg per 1ml, and get it.
Preparation of test solution: Take about 0.1g powder (passed through the No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 25ml of dilute ethanol, weigh it, and ultrasonically treat it (power 400W, frequency 40kHz) for 30 minutes, let it cool, and then weigh it. Use dilute ethanol to make up the lost weight, shake well, filter, and take the filtrate to get it.
Detection method: Precisely draw 10μl each of the reference solution and test solution, and inject it into the HPLC instrument for determination.
This product is calculated as a dry product and contains no less than 0.70% of mangiferin (C19H18O11).
Anemarrhena saponins BII is determined by HPLC.
Chromatographic conditions and system suitability test: use Octylsilane bonded silica gel as filler, acetonitrile-water (25: 75) as mobile phase, ELSD as detection. The number of theoretical plates should not be less than 10,000 calculated on the peak of Anemarrhena saponins BII.
Preparation of the reference solution: Take an appropriate amount of Anemarrhena saponins BII reference substance, accurately weigh it, and add 30% acetone to make a solution containing 0.50 mg per 1 ml.
The preparation of test solution: Take about0.15g powder (pass the No. 3 sieve) , accurately weigh it, place it in a stoppered conical flask, add 25ml 30% acetone, weigh it, and ultrasonically treat it (power 400W, frequency 40kHz) for 30 minutes, take it out, let it cool, and then weigh it. Make up the lost weight with 30% acetone and shake it well. Filter, take the filtrate, and get it.
Detection method: precisely draw 5μl,10ul reference solution and 5-10μl test solution respectively, inject it into the HPLC instrument for determination, and calculate with the External standard two-point method logarithmic equation and get it.
This product is calculated as a dry product and contains not less than 3.0% Anemarrhena saponins BII (C45H76O19).