[Content determination] According to HPLC determination.
Chromatographic conditions and system suitability test: use ODS as filler, methanol-0.1% acetic acid solution (85:15) as mobile phase, detection wavelength as 352nm.The number of theoretical plates should not be less than 3000 calculated on the peak of α-Terthiophene(C12H8S3).
Preparation of reference substance solution: Take an appropriate amount of α-Terthiophene reference substance, accurately weigh it, and add methanol to make a solution containing 0.26 mg per 1 ml.
Preparation of the test solution: Take about 0.25g powder (pass through the No. 4 sieve) , accurately weigh it, place it in a stoppered conical flask, accurately add 10ml methanol, tightly stopper, weigh it, ultrasonically treat it (power 300W, frequency 40kHz) for 30 minutes, let it cool, and then weigh it. Make up the lost weight with methanol, shake well, filter, and take the filtrate to get it.
Detection method: Precisely draw 10μl each of the reference solution and test solution, and inject it into the HPLC instrument for determination.




