Detection method of geniposide content in gardenia
[Content determination] According to HPLC determination.
Chromatographic conditions and system suitability test: Use ODS filler; acetonitrile-water (15:85) as the mobile phase; the detection wavelength as 238nm. The number of theoretical plates should not be less than 1500 based on the peak of geniposide.
Preparation of reference substance solution: Take an appropriate amount of geniposide reference substance, accurately weigh it, add methanol to make a solution containing 30ug per 1ml, then it is ready.
Preparation of the test solution: Take about 0.1g of the powder (passed through the No. 4 sieve), accurately weigh it, and place it in a conical flask with a stopper, add 25ml of methanol accurately, weigh it, ultrasonically treat it for 20 minutes, and let it cool. Check the weight again, make up the lost weight with methanol, shake well, and filter. Accurately measure 10ml of the continuous filtrate, put it in a 25ml measuring flask, add methanol to the mark, shake well, then get it.
Detection method: Precisely draw 10ul each of the reference solution and test solution, and inject it into the HPLC instrument for determination.
This product is calculated as a dry product and contains geniposide (C17H24O10) not less than 1.8%.